Monthly Archives: 1 月 2015

尋找所需要的fasta序列，以curcuma longa為例

利用blastn的方式選取不同種的curcuma

在存檔的地方選有aligned的選項，載下來的檔案是txt檔，可利用更改副檔名的方式，將它存為fasta檔

再利用MEGA進行一次Alignment

進入後

在phylogeny的地方接選取Bootstrap method

即可得到

Q1: Install the melanie view (Viewer_v502.exe):

Analyze the gel on folder \gels\94-0002.mel, 94-0005.mel, and ECOLI.mel

1.Which is the most abundant protein in gel ECOLI.mel in the region range from pH 5.5-6, MW 16k-32k Da, please answer with the protein name, official name, accession number, %vol, measured pI/MW, theoretic pI/MW

2.Set ECOLI as reference gel, select as group on three gels, and analyze the expression ratio of above protein in the three gels. (ECOLI : 94-0005 : 94-0002)

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Q2: A MS/MS data of spotX from bacteria was obtain in next slide with m/z lists.

–what this protein is (protein name, gene name, accession number)