1.下載MEGA
2.找一個植物的物種 (ex:Arecaceae 棕櫚科)
3.至NCBI,選nucleotide,關鍵字搜尋”Arecaceae rbcl” (rbcl為基因名)
4.任意勾選10個結果,並儲存成FASTA檔
5.打開MEGA後開啟FASTA檔
1)將序列Alignment
2)將FASTA轉檔
5.分析:1)Neighbor-Joining Tree
2)Maximum Parsimony Tree
*要選Bootstrap method
1)
2)
3.選一個物種Place Root Branch